Atomic Absorption Spectrophotometer (AAS)

Instrument Details:

Model:  Solar M-6

Make:  ThermoFisher

Working Principle:

Atomic Absorption Spectroscopy (AAS) determines the concentrations of metals in liquid samples. It is a process involving the absorption by free atoms of an element of light at a wavelength specific to that element. Atomic absorption spectroscopy is a method for quantitative determination of metals present in sample. In their elemental form, metals will absorb ultraviolet (UV) light when they are excited by heat. Each metal has a characteristic wavelength that will be absorbed.

Applications:

•  Analysis of trace elements in soil, water, fish samples, feed etc.

User Instructions:

If materials are given in raw form then about 50gm of sample is required and in case of dried or grinded sample the sample amount requirement is about 10gm. The sample is prepared through acid digestion and glass filtration process accordingly. For analysis of water sample the required amount is 100ml for each.

Contact us:

Contact: 91-674-2465421

E-mail ID: subhendu66@rediffmail.com

Atomic Absorption Spectrophotometer Charges Including GST: 

 Additional Information

1. How many days before the user Scientist is to be contacted?

   At least 2 weeks.

2. How the sample is to be prepared?

   The sample preparation is done as per the method of Paul et. al. (2014). The mineral assay is to be done as per (AOAC, 2005) in Flame atomic absorption spectrophotometer (Thermo Fisher).

3. How many days are required to analyse the sample?

   For acid digestion method, it takes 2-3days and 1 day for analysis in AAS.

4. Charges for sample analysis?

 Charge for sample analysis with report is Rs 500.00; which includes the sample preparation also. The raw tissue sample of about 50-60 g is required. If the sample is dried and grinded then 10 g tissue sample is required.

5. How the data is stored?

   After analysis the result is stored in the respective software (SOLAAR).

Bioinformatics Data Analysis and Training Facility

Instrument Details:  

Model: Z-HP-840 & Z-HP-420 Workstation    

Make: HP

Applications:  

• Bioinformatics data analysis using open software and CLC Genomics Workbench

 User Instructions: Raw data is required.  Analysis will be done by us.

 Contact us:

 Contact: 91-674-2465421

 E-mail ID: pdas77@hotmail.com

 Bioinformatics Facility Charges Including GST:

DNA Workstation

Instrument Details:

Model: QIAcube

Make: Qiagen

Working Principle:

Applications:

• Automated DNA /RNA isolation from different fish/animal tissues

User Instructions: NA

Contact us:

Contact: 91-674-2465421

E-mail ID: pdas77@hotmail.com

DNA Workstation Charges Including GST:

Gas Chromatograph (GC)

Instrument Details:

Model: -

Make: Shimadzu

Working Principle:

Applications: 

• Analysis of Fatty Acid profile of Fish tissue.

User Instructions:

Contact us:

Contact: 91-674-2465421

E-mail ID: knmohanta@gmail.com

Gas Chromatograph (GC) Charges Including GST:

Gas Liquid Chromatograph (GC)

Instrument Details:

Model: Clarus 480

Make: Perkin-Elmer

Working Principle: 

The principle in gas chromatography involves the separation of the components of the sample under test due to partition in between gaseous mobile phase and stationary liquid phase. The components partitioned into gas come out first while other comes later.

Gas chromatography runs on the principle of partition chromatography for separation of components. In terms of stationary and mobile phases it is categorized under gas-liquid type of chromatography i.e. stationary phase is a liquid layer supported over a stationary phase while the mobile phase is an inert and stable gas.

Applications:

• Analysis of Fatty Acid profile of Fish tissue.

User Instructions:

The raw tissue sample of about 50-60 g is required. If the sample is freeze dried then 20 g tissue sample is required.

Contact us:

Contact: 91-674-2465421

E-mail ID: bnpaulcifa@gmail.com

Gas Liquid Chromatograph (GC) Charges Including GST: 

Additional Information

1. How many days before the user Scientist is to be contacted?

   At least 3 weeks. 

2. How the sample is to be prepared?

Pooled samples to be  extracted for fatty acid analysis following the method of Folch et al., (1957) using chloroform and methanol (2:1, v/v) solvent system that contained 0.01% butylated hydroxyl anisole as an antioxidant. Fatty acid methyl esters (FAMEs) are prepared by the transmethylation with boron trifluoride (BF₃) in methanol from lipids fraction according to Metcalfe et al., (1966).

3. How many days are required to analyse the sample?

  For preparation of Fatty Acid Methyl Ester (FAME), it takes 2-3 days and 1 day for analysis in GC. 

4. Charges for sample analysis?

Charge for sample analysis with report is Rs 3000.00; which includes the sample preparation also. The raw tissue sample of about 50-60 g is required. If the sample is freeze dried then 20 g tissue sample is required. If the sample is already processed as Fatty acid methyl ester form (FAME) then the charge would be Rs. 1000.00.

5. How the data is stored?

 After analysis the chromatogram is stored in the software (TC Navigator) of the machine.